Scent marking is a frustrating issue that many pet owners face on a daily basis, and in pooches of both sexes. Although getting a dog fixed often does away with the problem, exceptions definitely exist.
Yes, They Can
Urine marking in dogs is often a hormonal action, whether it's caused by territorial feelings or the urge to find a mate. Spaying a female dog calms down those racing hormones, but if the surgery is performed on an animal who is so used to those feelings and the routine of heat cycles, the issues could continue. If your pooch has been spraying for a long time, it might not be easy for her to just stop on a dime. Female dogs can indeed scent mark post-spaying.
Other Scent Marking Causes
Not all marking is driven by hormones. If your dog's marking isn't fueled by hormones, spaying probably won't make a difference. Some canines scent mark because they're practically bursting at the seams with enthusiasm, or perhaps even because they're full of apprehensive nerves due to the presence of a couple new cats in the home -- especially if those felines aren't fixed. Marking can sometimes even signify the emergence of a medical problem, whether urinary incontinence or bacterial infection. The only way to know exactly what's going on is by taking your fluff ball to the vet -- pronto.