Canine Ovulation Testing

Most canine ovulation tests determine the dog's luteinizing hormone levels.
Dobermann image by Manuel Weber from

In regular dog breeding or in cases of artificial insemination, knowing when a dog is ovulating helps speed the process. Tests are available to determine if a bitch is in the prime period of her cycle to achieve pregnancy.


Canine ovulation tests check the dog’s luteinizing hormone levels to determine if she is ovulating. Blood samples are tested daily at a veterinarian’s office for the greatest accuracy.


Pregnancy is most likely to be established within three to seven days of a surge of the luteinizing hormone in the dog’s body, according to the American Rottweiler Club. The male’s sperm can survive in the bitch’s body for about five days.

Signs of Ovulation

Owners might notice changes in behavior, as well as vaginal discharge containing blood, when a dog is preparing to ovulate. These changes are set off by changes in the estrogen level, which are not as reliable as an LH test for predicting ovulation.


Ovulation pads also can be used to predict ovulation. The pads change color when soaked with vaginal fluid from a bitch in which elevated luteinizing hormone is detected. Kits also are available for home use that test blood for progesterone levels to predict ovulation.